Journal: Endocrinology

Article Title: Determination of three isoforms of the receptor activator of nuclear factor-kappaB ligand and their differential expression in bone and thymus.

doi: 10.1210/endo.142.4.8070

Figure Lengend Snippet: FIG. 1. Alignment of RANKL 1, RANKL 2, and RANKL 3 cDNA sequences. Sequences representing predicted transmembrane domains in RANKL 1 and RANKL 2 are shaded. Primer sets used for cloning of RANKL cDNA (R-5; R-3) and specific detection of RANKL 1 (R1-5; R-3), RANKL 2 (R2-5; R-3), and RANKL 3 (R3-5; R-3) are indicated by arrows.

Article Snippet: RANKL 3 complementary DNA (cDNA) fragments were used as probe and labeled with [32P]deoxycycidine triphosphate (Amersham Pharmacia Biotech).

Techniques: Cloning

Journal: Endocrinology

Article Title: Determination of three isoforms of the receptor activator of nuclear factor-kappaB ligand and their differential expression in bone and thymus.

doi: 10.1210/endo.142.4.8070

Figure Lengend Snippet: FIG. 6. Immunofluorescence study on localization of RANKL 1, RANKL 2, and RANKL 3 in NIH3T3 cells transfected with pMIKHygB vector (A and B), the vector with RANKL 1 cDNA (C and D), the vector with RANKL 2 cDNA (E and F), or the vector with RANKL 3 cDNA (G and H). A, C, E, and G represent cells treated with the RANKL antiserum; and B, D, F and H represent cells treated with the preimmune serum. Findings of clones RANKL 1-2, RANKL 2-1, RANKL 3-2, and HV are presented. In C, E, and G, a positive signal of RANKL protein is observed; but in A, no clear signal is seen. In B, D, F, and H, no specific signal is seen. Scale bars, 20 mm.

Article Snippet: RANKL 3 complementary DNA (cDNA) fragments were used as probe and labeled with [32P]deoxycycidine triphosphate (Amersham Pharmacia Biotech).

Techniques: Immunofluorescence, Transfection, Plasmid Preparation, Clone Assay

Journal: Endocrinology

Article Title: Determination of three isoforms of the receptor activator of nuclear factor-kappaB ligand and their differential expression in bone and thymus.

doi: 10.1210/endo.142.4.8070

Figure Lengend Snippet: FIG. 5. Northern hybridization of NIH3T3 cells transfected with RANKL 1, RANKL 2, or RANKL 3 cDNA cloned into pMIKHygB expression vector. The lane HV represents the basal expression level of RANKL mRNA in NIH3T3 cells transfected with pMIKHygB vector without insert cDNA (exposed for 16 h).

Article Snippet: RANKL 3 complementary DNA (cDNA) fragments were used as probe and labeled with [32P]deoxycycidine triphosphate (Amersham Pharmacia Biotech).

Techniques: Northern Blot, Hybridization, Transfection, Clone Assay, Expressing, Plasmid Preparation